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KMID : 0603519970020020113
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Journal of Korean Association of Cancer Prevention
1997 Volume.2 No. 2 p.113 ~ p.121
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In vitro Antimutagenic and Anticancer Effects of Kimchi Fractions
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Cho Eun-Ju
Lee Sook-Hee
Lee Sun-Mi
Park Kun-Young
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Abstract
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Antimutagenic and anticancer effects of kimchi fractions were studied by using Salmonella typhimurium TA 100 and HL-60 human leukemia cells, respectively. The chinese cabbage kimchi(4 day fermented at 15℃) was fractionated into 7 groups, methanol extract, hexane fraction(fr.), methanol soluble fr., dichloromethane fr., ethylacetate fr., butanol fr. and aqueous fr. The dichloromethane fr. and hexane fr. of the kimchi exhibited strong antimutagenic activities compared to other fractionated samples, especially dichloromethane fr. inhibited the mutagenicity of aflatoxin B1 (AFB1) by more than 90% in the Ames test. The kimchi fractions inhibited the survival or growth of HL-60 human leukemia cells in the sulforhodamine B(SRB) assay, 3-(4,5-dimethylthiazol)-2, 5-diphenyl-tetrazolium bromide(MTT) assay and growth inhibition test. All the kimchi fractions inhibited survival of HL-60 leukemia cells more than 50% in the addition concentration of 0.2 mg/assay, in particular dichloromethane fr. showed the strongest inhibitory effect (more than 90%) among them. Moreover dichloromethane fr. of the kimchi induced atoptosis in HL-60 human leukemia cells by the DNA fragmentation assay. These results suggested that the kimchi fractions, especially dichloromethane fr. exerted the survival or growth inhibitory effect on HL-60 human leukemia cells as well as antimutagenic activity, and the dichloromethane fr. also induced apoptosis in the leukemia cells.
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KEYWORD
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Kimchi, Antimutagenicity, SRB, MTT, Apoptosis, HL-60 cells
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